Optofluidic cell manipulation for a biological microbeam.
نویسندگان
چکیده
This paper describes the fabrication and integration of light-induced dielectrophoresis for cellular manipulation in biological microbeams. An optoelectronic tweezers (OET) cellular manipulation platform was designed, fabricated, and tested at Columbia University's Radiological Research Accelerator Facility (RARAF). The platform involves a light induced dielectrophoretic surface and a microfluidic chamber with channels for easy input and output of cells. The electrical conductivity of the particle-laden medium was optimized to maximize the dielectrophoretic force. To experimentally validate the operation of the OET device, we demonstrate UV-microspot irradiation of cells containing green fluorescent protein (GFP) tagged DNA single-strand break repair protein, targeted in suspension. We demonstrate the optofluidic control of single cells and groups of cells before, during, and after irradiation. The integration of optofluidic cellular manipulation into a biological microbeam enhances the facility's ability to handle non-adherent cells such as lymphocytes. To the best of our knowledge, this is the first time that OET cell handling is successfully implemented in a biological microbeam.
منابع مشابه
Optofluidic Devices for Droplet and Cell Manipulation
Optofluidic Devices for Droplet and Cell Manipulation
متن کاملApplication of Nano-Contact Mechanics Models in Manipulation of Biological Nano-Particle: FE Simulation
Contact mechanics is related to the deformation study of solids that meet each other at one or more points. The physical and mathematical formulation of the problem is established upon the mechanics of materials and continuum mechanics and focuses on computations involving bodies with different characteristics in static or dynamic contact. Contact mechanics gives essential information for the s...
متن کاملHigh-throughput optofluidic system for the laser microsurgery of oocytes.
This study combines microfluidics with optical microablation in a microscopy system that allows for high-throughput manipulation of oocytes, automated media exchange, and long-term oocyte observation. The microfluidic component of the system transports oocytes from an inlet port into multiple flow channels. Within each channel, oocytes are confined against a microfluidic barrier using a steady ...
متن کاملERRATUM: Integrated microfluidic device for single-cell trapping and spectroscopy
Optofluidic microsystems are key components towards lab-on-a-chip devices for manipulation and analysis of biological specimens. In particular, the integration of optical tweezers (OT) in these devices allows stable sample trapping, while making available mechanical, chemical and spectroscopic analyses.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Review of scientific instruments
دوره 84 1 شماره
صفحات -
تاریخ انتشار 2013